Rat Osteopontin, OPN ELISA Kit

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Blood coagulation
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Home > Product > ELISA Kit > Rat Osteopontin, OPN ELISA Kit

Rat Osteopontin, OPN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.25ng/ml

Detection range: 0.5-200ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.25ng/ml

Detection range: 0.5-200ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Osteopontin

Code

E0273Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.25ng/ml

Detection range

0.5-200ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

UniProt accession

P08721

MASS（Da）

34,963

GeneID

25353

Synonyms

Bone sialoprotein 1;Osteopontin;Secreted phosphoprotein 1;SPP 1;SPP1;SPP-1

Gene names

SPP1

Research area

Metabolism

Target protein

SPP1

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E0273Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	2.234	2.178	2.123
	2.123
60ng/ml	1.674	1.674	1.619
	1.674
30ng/ml	0.963	0.995	0.94
	1.027
15ng/ml	0.667	0.717	0.662
	0.767
7.5ng/ml	0.421	0.45	0.395
	0.479
0ng/ml	0.06	0.055	0
	0.05

Precision

We measured random samples of E0273Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%

Protocols

> Protocol Booklet

References (9)

Evaluation of the Effects of Topical Ellagic Acid and Graft Application on Bone Regeneration: an Experimental Study

Journal: J Oral Maxillofac Res. 2022 Jun 30;13(2):e3

Irisin alleviates liver ischemia-reperfusion injury by inhibiting excessive mitochondrial fission, promoting mitochondrial biogenesis and decreasing oxidative stress

Journal: Redox Biol. 2019 Jan;20:296-306.

Vitamin C modulates adrenaline-augmented gastric injury via cardiac troponin/creatine kinase pathway in Wistar rats

Journal: Iran J Basic Med Sci. 2023;26(7):843-850.

Serological response and breakthrough infection after COVID-19 vaccination in patients with cirrhosis and post-liver transplant

Journal: Hepatol Commun. 2023 Oct 18;7(11):e0273.

Seizure burden fluctuates with the female reproductive cycle in a mouse model of chronic temporal lobe epilepsy

Journal: Exp Neurol. 2020 Dec;334:113492.

View all publications for the product

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Associated products

Datasheet

Size

Code

Price

48T

E0273Ra

$320.00
96T

E0273Ra

$458.00

Add to Cart

Lead time: Within two weeks

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