Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Rat Bone Morphogenetic Protein 2, BMP-2 ELISA Kit

Rat Bone Morphogenetic Protein 2, BMP-2 ELISA Kit

Rat Bone Morphogenetic Protein 2, BMP-2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.51ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 2.51ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Bone Morphogenetic Protein 2

Code

E0276Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.51ng/L

Detection range

5-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Induces cartilage and bone formation. Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4.

UniProt accession

MASS(Da)

44,383

GeneID

29373

Synonyms

BMP 2;BMP2;BMP-2;BMP-2A;Bone morphogenetic protein 2;Bone morphogenetic protein 2A

Gene names

BMP2

Research area

Cardiovascular; Signal Transduction

Target protein

BMP2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0276Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 1.854 1.856 1.817
1.857
400ng/L 0.887 0.883 0.844
0.879
200ng/L 0.571 0.596 0.557
0.62
100ng/L 0.24 0.236 0.197
0.233
50ng/L 0.079 0.107 0.068
0.135
0ng/L 0.028 0.039 0
0.05

Precision

We measured random samples of E0276Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 699.4 15.62 2.2
Intra-Assay 2 18 199.2 9.49 4.8
Intra-Assay 3 18 338.7 19.46 5.7

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E0276Ra

    $320.00

  • 96T

    E0276Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up