Rat Matrix Metalloproteinase 2, MMP2 ELISA Kit

Product

OneStep ELISA kit
Primacu ELISA kit
Antibodies
Biochemicals
Recombinant proteins

Service

Custom ELISA assay services
Bulk quote

Research area

Blood coagulation
Metabolism
Cancer
Microbiology
Cardiovascular
Neuroscience
Cell biology
Signal transduction
Developmental biology
Stem cells
Epigenetics and nuclear signaling
Tags & cell markers
Immunology

Resources

Protocols
FAQs
Product brochure
Promotion
Troubleshooting guides
About ELISAs

About us

Company profile
Worldwide distributors
Manufacturing service

Home > Product > ELISA Kit > Rat Matrix Metalloproteinase 2, MMP2 ELISA Kit

Rat Matrix Metalloproteinase 2, MMP2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.092ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.092ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Matrix Metalloproteinase 2

Code

E0315Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.092ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity). PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta3 on the surface of blood vessels (By similarity).

UniProt accession

P33436

MASS（Da）

74,149

GeneID

81686

Synonyms

72 kDa gelatinase;72 kDa type IV collagenase;Gelatinase A;Matrix Metalloproteinase-2;MMP 2;MMP2;MMP-2;PEX

Gene names

MMP2

Research area

Cancer

Target protein

MMP2

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E0315Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.154	2.219	2.162
	2.283
20ng/ml	1.259	1.357	1.301
	1.455
10ng/ml	0.71	0.802	0.746
	0.894
5ng/ml	0.438	0.442	0.386
	0.446
2.5ng/ml	0.318	0.318	0.262
	0.318
0ng/ml	0.048	0.056	0
	0.064

Precision

We measured random samples of E0315Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	14.8	0.78	5.3
Intra-Assay	2	18	25.5	1.01	4
Intra-Assay	3	18	4.3	0.24	5.7

Protocols

> Protocol Booklet

References (2)

Pomegranate peel extract, N-Acetylcysteine and their combination with Ornipural alleviate Cadmium-induced toxicity in rats

Journal: J Vet Med Sci. 2023 Sep 7;85(9):990-997.

Activation of the Mas receptors by AVE0991 and MrgD receptor using alamandine to limit the deleterious effects of Ang II-induced hypertension

Journal: Fundam Clin Pharmacol. 2023 Feb;37(1):60-74.

Customer reviews and Q&As

No reviews

Associated products

Datasheet

Size

Code

Price

48T

E0315Ra

$320.00
96T

E0315Ra

$458.00

Add to Cart

Lead time: Within two weeks

Custom quote | Bulk request

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.