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Home > Product > ELISA Kit > Bovine Insulin Like Growth Factor Binding Protein 3, IGFBP3 ELISA Kit

Bovine Insulin Like Growth Factor Binding Protein 3, IGFBP3 ELISA Kit

Bovine Insulin Like Growth Factor Binding Protein 3, IGFBP3 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.24ng/ml

Detection range: 0.5-200ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 0.24ng/ml

Detection range: 0.5-200ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Insulin Like Growth Factor Binding Protein 3

Code

E0017Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.24ng/ml

Detection range

0.5-200ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

UniProt accession

MASS(Da)

31,570

GeneID

282261

Synonyms

IBP-3;IGF-binding protein 3;IGFBP 3;IGFBP3;IGFBP-3;Insulin-like growth factor-binding protein 3

Gene names

IGFBP3

Research area

Cell Biology; Signal Transduction; Cardiovascular; Metabolism; Cancer

Target protein

IGFBP3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Bovine Insulin Like Growth Factor Binding Protein 3, IGFBP3 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
120ng/ml 2.434 2.399 2.369
2.365
60ng/ml 1.454 1.345 1.315
1.236
30ng/ml 0.576 0.624 0.594
0.673
15ng/ml 0.416 0.422 0.391
0.427
7.5ng/ml 0.119 0.147 0.117
0.176
0ng/ml 0.041 0.031 0
0.02

Precision

We measured random samples of E0017Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 18.2 0.78 4.3
Intra-Assay 2 18 79.7 2.38 3
Intra-Assay 3 18 50.6 2.88 5.7

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 96T

    $458.00

  • 48T

    $320.00

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Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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