Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
CD3 (cluster of differentiation 3) is a protein complex and T cell co-receptor that is involved in activating both the cytotoxic T cell (CD8+ naive T cells) and T helper cells (CD4+ naive T cells). It is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0221Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ng/ml | 2.218 | 2.272 | 2.23 |
| 2.325 | |||
| 6ng/ml | 1.426 | 1.331 | 1.289 |
| 1.236 | |||
| 3ng/ml | 0.585 | 0.64 | 0.598 |
| 0.695 | |||
| 1.5ng/ml | 0.336 | 0.359 | 0.317 |
| 0.381 | |||
| 0.75ng/ml | 0.181 | 0.166 | 0.124 |
| 0.15 | |||
| 0ng/ml | 0.039 | 0.042 | 0 |
| 0.045 |
Precision
We measured random samples of E0221Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 2.1 | 0.09 | 4.4 |
| Intra-Assay | 2 | 18 | 5.6 | 0.33 | 5.9 |
| Intra-Assay | 3 | 18 | 6.4 | 0.18 | 2.8 |
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