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Home > Product > ELISA Kit > Rat Desmin, DES ELISA Kit

Rat Desmin, DES ELISA Kit

Rat Desmin, DES ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.28ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.28ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Desmin

Code

E0355Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.28ng/ml

Detection range

0.5-150ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin.

UniProt accession

MASS(Da)

53,457

GeneID

64362

Synonyms

DES;Desmin

Gene names

DES

Research area

Others

Target protein

DES

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0355Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
80ng/ml 2.149 2.112 2.083
2.076
40ng/ml 1.412 1.367 1.338
1.321
20ng/ml 0.623 0.607 0.578
0.591
10ng/ml 0.382 0.376 0.347
0.37
5ng/ml 0.182 0.179 0.15
0.176
0ng/ml 0.02 0.029 0
0.038

Precision

We measured random samples of E0355Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 62.4 2.16 3.5
Intra-Assay 2 18 17.8 0.99 5.6
Intra-Assay 3 18 25 1.05 4.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0355Ra

    $320.00

  • 96T

    E0355Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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