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Home > Product > ELISA Kit > Rat Macrophage Inflammatory Protein 1Α, MIP1A ELISA Kit

Rat Macrophage Inflammatory Protein 1Α, MIP1A ELISA Kit

Rat Macrophage Inflammatory Protein 1Α, MIP1A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.11ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 3.11ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Macrophage Inflammatory Protein 1alpha

Code

E0369Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.11ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.

UniProt accession

MASS(Da)

10,335

GeneID

25542

Synonyms

C-C motif chemokine 3;CCL 3;CCL3;Macrophage inflammatory protein 1-alpha;MIP-1-alpha;Small-inducible cytokine A3

Gene names

CCL3

Research area

Immunology

Target protein

CCL3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0369Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.118 2.179 2.106
2.24
400ng/L 1.347 1.329 1.257
1.311
200ng/L 0.838 0.813 0.74
0.787
100ng/L 0.4 0.417 0.345
0.435
50ng/L 0.254 0.287 0.215
0.321
0ng/L 0.078 0.073 0
0.067

Precision

We measured random samples of E0369Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 550.1 15.08 2.7
Intra-Assay 2 18 246.2 10.91 4.4
Intra-Assay 3 18 109.4 6.58 6

Protocols

References

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  • Size

    Code

    Price

  • 48T

    E0369Ra

    $320.00

  • 96T

    E0369Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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