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Home > Product > ELISA Kit > Rat Leptin, LEP ELISA Kit

Rat Leptin, LEP ELISA Kit

Rat Leptin, LEP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.05ng/ml

Detection range: 0.1-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.05ng/ml

Detection range: 0.1-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Leptin

Code

E0561Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.05ng/ml

Detection range

0.1-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways (By similarity) (PubMed: 11677594). In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity (By similarity) (PubMed: 11460888, PubMed: 10482243, PubMed: 9003024, PubMed: 11677594). In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic Peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic Peptides (By similarity) (PubMed: 10482243, PubMed: 9003024). In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption (By similarity). Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways (By similarity). May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression (By similarity). Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of Matrix Metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) (PubMed: 11460888). In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells (Probable). Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 wich promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38 (By similarity). In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses (By similarity). Increases CD4(+)CD25(-) T-cell proliferation and reduces autophagy during TCR (T-cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation (By similarity).

UniProt accession

MASS(Da)

18,866

GeneID

25608

Synonyms

LEP;LEPD;LEPTIN;OB;Obesity;Obesity factor;OBS

Gene names

LEPTIN

Research area

Signal Transduction; Neuroscience; Metabolism

Target protein

LEPTIN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0561Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.192 2.111 2.067
2.029
6ng/ml 1.672 1.682 1.638
1.691
3ng/ml 1.103 1.119 1.075
1.135
1.5ng/ml 0.665 0.693 0.649
0.721
0.75ng/ml 0.443 0.469 0.425
0.496
0ng/ml 0.035 0.044 0
0.053

Precision

We measured random samples of E0561Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.8 0.18 4.6
Intra-Assay 2 18 2.4 0.17 7
Intra-Assay 3 18 1 0.06 6.5

Protocols

References (11)

Voluntary exercise could reduce sperm malformations by improving hypothalamus-hypophysis-gonadal axis and kisspeptin/leptin signaling in type 2 diabetic rats
Evaluation of The Effects of Carob ( Ceratonia Siliqua L.) Fruits on the Puberty of Rats
HSP70, adiponectin, leptin, pro-inflammatory cytokines and metabolic hormones of heat-stressed broilers fed herbal supplements
Potential role of green tea extract and epigallocatechin gallate in preventing bisphenol A-induced metabolic disorders in rats: Biochemical and molecular evidence
Black yeast beta glucan for insulin resistance prevention through IL-33, ST2 and leptin Level: An In vivo study of an obesity model using Sprague dawley rats
View all publications for the product

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  • Size

    Code

    Price

  • 48T

    E0561Ra

    $320.00

  • 96T

    E0561Ra

    $458.00

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Lead time: Within two weeks

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