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Home > Product > ELISA Kit > Rat Cytochrome P450 7A1, CYP7A1 ELISA Kit

Rat Cytochrome P450 7A1, CYP7A1 ELISA Kit

Rat Cytochrome P450 7A1, CYP7A1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.17ng/ml

Detection range: 0.03-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.17ng/ml

Detection range: 0.03-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Cytochrome P450 7A1

Code

E0915Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.17ng/ml

Detection range

0.03-90ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols) (PubMed: 1694852, PubMed: 2335522). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed: 1694852, PubMed: 2335522). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis (PubMed: 1694852, PubMed: 2335522). 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives (By similarity).

UniProt accession

MASS(Da)

56,883

GeneID

25428

Synonyms

24-hydroxycholesterol 7-alpha-hydroxylase;Cholesterol 7-alpha-hydroxylase;Cholesterol 7-alpha-monooxygenase;CYP7A1;CYPVII;Cytochrome P450 7A1

Gene names

CYP7A1

Research area

Cardiovascular

Target protein

CYP7A1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0915Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/ml 2.591 2.593 2.547
2.595
24ng/ml 1.444 1.439 1.393
1.433
12ng/ml 0.884 0.914 0.868
0.945
6ng/ml 0.521 0.461 0.415
0.401
3ng/ml 0.251 0.248 0.202
0.245
0ng/ml 0.033 0.046 0
0.059

Precision

We measured random samples of E0915Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 14.8 0.68 4.6
Intra-Assay 2 18 19.6 0.51 2.6
Intra-Assay 3 18 6.8 0.41 6.1

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0915Ra

    $320.00

  • 96T

    E0915Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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