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Home > Product > ELISA Kit > Rat Insulin Receptor Substrate 1, IRS-1 ELISA Kit

Rat Insulin Receptor Substrate 1, IRS-1 ELISA Kit

Rat Insulin Receptor Substrate 1, IRS-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.13ng/ml

Detection range: 3-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 2.13ng/ml

Detection range: 3-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Insulin Receptor Substrate 1

Code

E0919Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.13ng/ml

Detection range

3-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.

UniProt accession

MASS(Da)

131,178

GeneID

25467

Synonyms

Insulin receptor substrate 1;IRS 1;IRS1;IRS-1;pp185

Gene names

IRS1

Research area

Tags & Cell Markers; Signal Transduction; Neuroscience; Metabolism

Target protein

IRS1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0919Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.287 2.295 2.257
2.302
20ng/ml 1.942 1.841 1.803
1.739
10ng/ml 1.212 1.251 1.213
1.29
5ng/ml 0.625 0.665 0.628
0.706
2.5ng/ml 0.348 0.348 0.31
0.348
0ng/ml 0.043 0.038 0
0.033

Precision

We measured random samples of E0919Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.1 0.28 5.5
Intra-Assay 2 18 7.3 0.4 5.4
Intra-Assay 3 18 12 0.65 5.4

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0919Ra

    $320.00

  • 96T

    E0919Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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