Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Rat Apelin, AP ELISA Kit

Rat Apelin, AP ELISA Kit

Rat Apelin, AP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.524ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.524ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Apelin

Code

E1026Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.524ng/mL

Detection range

0.1-40ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Endogenous ligand for the apelin receptor (APLNR) (PubMed: 11336787, PubMed: 11359874, PubMed: 26611206). Drives internalization of the apelin receptor (PubMed: 11359874). Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR (PubMed: 11336787). Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis (By similarity). Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates (By similarity). Plays a role in early coronary blood vessels formation (By similarity). Mediates myocardial contractility in an ERK1/2-dependent manner (PubMed: 26611206). May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior (PubMed: 10617103, PubMed: 11359874).

UniProt accession

MASS(Da)

8,677

GeneID

58812

Synonyms

Apelin;Apelin-13;Apelin-28;Apelin-31;Apelin-36;APJ endogenous ligand;APLN

Gene names

APLN

Research area

Others

Target protein

APLN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1026Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/mL 2.15 2.198 2.153
2.246
12ng/mL 1.367 1.25 1.205
1.134
6ng/mL 0.803 0.736 0.69
0.669
3ng/mL 0.426 0.403 0.357
0.38
1.5ng/mL 0.191 0.203 0.157
0.215
0ng/mL 0.063 0.046 0
0.028

Precision

We measured random samples of E1026Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 15.5 0.58 3.7
Intra-Assay 2 18 3.6 0.23 6.4
Intra-Assay 3 18 6.7 0.37 5.5

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E1026Ra

    $320.00

  • 96T

    E1026Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up