Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Rat Caspase 8, CASP8 ELISA Kit

Rat Caspase 8, CASP8 ELISA Kit

Rat Caspase 8, CASP8 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.023ng/mL

Detection range: 0.05-30ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.023ng/mL

Detection range: 0.05-30ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Caspase 8

Code

E1370Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.023ng/mL

Detection range

0.05-30ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood (By similarity). Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death (PubMed: 10197541). Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10 (By similarity). Binding to the adapter molecule FADD recruits it to either receptor TNFRSF6/FAS mediated or TNFRSF1A (PubMed: 10197541). The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation (By similarity). The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases (By similarity). Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC (By similarity). In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at 'Asp-325', which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response (By similarity). Also able to initiate pyroptosis by mediating cleavage and activation of gasdermin-D (GSDMD): GSDMD cleavage promoting release of the N-terminal moiety (Gasdermin-D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis (By similarity). Initiates pyroptosis following inactivation of MAP3K7/TAK1 (By similarity). Also acts as a regulator of innate immunity by mediating cleavage and inactivation of N4BP1 downstream of TLR3 or TLR4, thereby promoting cytokine production (By similarity). May participate in the Granzyme B (GZMB) cell death pathways (By similarity). Cleaves PARP1 (By similarity).

UniProt accession

MASS(Da)

55,339

GeneID

64044

Synonyms

CASP 8;CASP8;CASP-8;Caspase-8;Caspase-8 subunit p10;Caspase-8 subunit p18

Gene names

CASP8

Research area

Cell Biology; Cancer; Metabolism

Target protein

CASP8

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1370Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/mL 2.384 2.259 2.195
2.133
8ng/mL 1.724 1.771 1.707
1.818
4ng/mL 1.096 1.108 1.044
1.12
2ng/mL 0.673 0.704 0.64
0.736
1ng/mL 0.412 0.403 0.339
0.394
0ng/mL 0.065 0.064 0
0.063

Precision

We measured random samples of E1370Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.6 0.1 6.5
Intra-Assay 2 18 4.3 0.31 7.4
Intra-Assay 3 18 9.3 0.58 6.3

Protocols

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E1370Ra

    $320.00

  • 96T

    E1370Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up