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Home > Product > ELISA Kit > Human Toll-like Receptor 7, TLR7 ELISA Kit

Human Toll-like Receptor 7, TLR7 ELISA Kit

Human Toll-like Receptor 7, TLR7 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 5.32ng/L

Detection range: 10-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 5.32ng/L

Detection range: 10-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Toll-Like Receptor 7

Code

E0334Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

5.32ng/L

Detection range

10-3500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Endosomal receptor that plays a key role in innate and adaptive immunity (PubMed: 14976261, PubMed: 32433612). Controls host immune response against pathogens through recognition of uridine-containing single strand RNAs (ssRNAs) of viral origin or guanosine analogs (PubMed: 31608988, PubMed: 27742543, PubMed: 12738885). Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction (PubMed: 27742543). In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce proinflammatory cytokines and interferons, respectively (PubMed: 27742543).

UniProt accession

MASS(Da)

120,922

GeneID

51284

Synonyms

TLR 7;TLR7;Toll-like receptor 7

Gene names

TLR7

Research area

Immunology; Signal Transduction; Epigenetics and Nuclear Signaling

Target protein

TLR7

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0334Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.204 2.2 2.13
2.196
1000ng/L 1.245 1.343 1.273
1.442
500ng/L 0.862 0.777 0.707
0.693
250ng/L 0.51 0.515 0.445
0.519
125ng/L 0.28 0.305 0.234
0.329
0ng/L 0.078 0.07 0
0.063

Precision

We measured random samples of E0334Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 818.4 40.89 5
Intra-Assay 2 18 1387.5 46.12 3.3
Intra-Assay 3 18 318.7 20.59 6.5

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 96T

    E0334Hu

    $458.00

  • 48T

    E0334Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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