Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Rat Monocarboxylate Transporter-1, MCT-1 ELISA Kit

Rat Monocarboxylate Transporter-1, MCT-1 ELISA Kit

Rat Monocarboxylate Transporter-1, MCT-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.058ng/ml

Detection range: 0.1-35ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.058ng/ml

Detection range: 0.1-35ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Monocarboxylate Transporter-1

Code

E1673Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.058ng/ml

Detection range

0.1-35ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.

UniProt accession

MASS(Da)

53,238

GeneID

25027

Synonyms

MCT 1;Monocarboxylate transporter 1;SLC16A1;Solute carrier family 16 member 1

Gene names

SLC16A1

Research area

Signal Transduction

Target protein

SLC16A1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1673Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/ml 2.171 2.107 2.067
2.042
10ng/ml 1.417 1.454 1.414
1.492
5ng/ml 0.651 0.705 0.664
0.759
2.5ng/ml 0.306 0.31 0.269
0.313
1.25ng/ml 0.136 0.166 0.125
0.195
0ng/ml 0.038 0.04 0
0.042

Precision

We measured random samples of E1673Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 10.4 0.46 4.4
Intra-Assay 2 18 7.8 0.38 4.9
Intra-Assay 3 18 3 0.1 3.3

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E1673Ra

    $320.00

  • 96T

    E1673Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up