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Home > Product > ELISA Kit > Rat Bone Morphogenetic Protein 10, BMP-10 ELISA Kit

Rat Bone Morphogenetic Protein 10, BMP-10 ELISA Kit

Rat Bone Morphogenetic Protein 10, BMP-10 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.52ng/L

Detection range: 1.03-300ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.52ng/L

Detection range: 1.03-300ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Bone Morphogenetic Protein 10

Code

E1699Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.52ng/L

Detection range

1.03-300ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. May reduce cell migration and cell matrix adhesion in breast cancer cell lines.

UniProt accession

MASS(Da)

47,767

GeneID

500245

Synonyms

BMP 10;BMP10;BMP-10;Bone morphogenetic protein 10

Gene names

BMP10

Research area

Signal Transduction; Cardiovascular

Target protein

BMP10

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1699Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
160ng/L 2.196 2.132 2.086
2.068
80ng/L 1.387 1.377 1.331
1.368
40ng/L 0.852 0.853 0.807
0.855
20ng/L 0.425 0.436 0.389
0.446
10ng/L 0.241 0.246 0.199
0.25
0ng/L 0.046 0.046 0
0.047

Precision

We measured random samples of E1699Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 119.3 5.27 4.4
Intra-Assay 2 18 28.8 1.99 6.9
Intra-Assay 3 18 41.7 2.03 4.9

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1699Ra

    $320.00

  • 96T

    E1699Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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