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Home > Product > ELISA Kit > Rat Α2 Macroglobulin, ALPHA2-MG ELISA Kit

Rat Α2 Macroglobulin, ALPHA2-MG ELISA Kit

Rat Α2 Macroglobulin, ALPHA2-MG ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.21μg/ml

Detection range: 0.5-230ug/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.21μg/ml

Detection range: 0.5-230ug/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

alpha2 Macroglobulin

Code

E1784Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.21μg/ml

Detection range

0.5-230ug/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a Peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

UniProt accession

MASS(Da)

163,785

GeneID

10091154524153

Synonyms

A2M;Alpha-2-M;Alpha-2-macroglobulin

Gene names

A2M

Research area

Immunology

Target protein

A2M

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1784Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
128μg/ml 2.482 2.447 2.395
2.412
64μg/ml 1.455 1.389 1.337
1.323
32μg/ml 0.944 0.918 0.866
0.892
16μg/ml 0.384 0.38 0.328
0.377
8μg/ml 0.259 0.253 0.201
0.246
0μg/ml 0.06 0.052 0
0.045

Precision

We measured random samples of E1784Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 52 2.54 4.9
Intra-Assay 2 18 71 2.52 3.6
Intra-Assay 3 18 15.1 0.78 5.2

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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