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Home > Product > ELISA Kit > Rat 14-3-3 Protein Epsilon, YWHAE ELISA Kit

Rat 14-3-3 Protein Epsilon, YWHAE ELISA Kit

Rat 14-3-3 Protein Epsilon, YWHAE ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

14-3-3 Protein Epsilon

Code

E1830Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.026ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm.

UniProt accession

MASS(Da)

29,174

GeneID

29753

Synonyms

14-3-3 protein epsilon;14-3-3E;Mitochondrial import stimulation factor L subunit;MSF L;YWHAE

Gene names

YWHAE

Research area

Neuroscience; Signal Transduction; Stem Cells

Target protein

YWHAE

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1830Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.361 2.341 2.281
2.321
6ng/mL 1.753 1.73 1.669
1.706
3ng/mL 1.308 1.213 1.152
1.117
1.5ng/mL 0.707 0.705 0.645
0.703
0.75ng/mL 0.423 0.423 0.362
0.422
0ng/mL 0.065 0.06 0
0.055

Precision

We measured random samples of E1830Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5 0.24 4.9
Intra-Assay 2 18 0.9 0.06 6.7
Intra-Assay 3 18 2.7 0.18 6.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1830Ra

    $320.00

  • 96T

    E1830Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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