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Home > Product > ELISA Kit > Rat Actin, Cytoplasmic 1, ACTB ELISA Kit

Rat Actin, Cytoplasmic 1, ACTB ELISA Kit

Rat Actin, Cytoplasmic 1, ACTB ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Actin, Cytoplasmic 1

Code

E1841Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.019ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.

UniProt accession

MASS(Da)

41,737

GeneID

81822

Synonyms

ACTB;Actin, cytoplasmic 1;Actin, cytoplasmic 1, N-terminally processed;Beta-actin

Gene names

ACTB

Research area

Others

Target protein

ACTB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1841Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.323 2.344 2.301
2.365
6ng/mL 1.681 1.596 1.553
1.511
3ng/mL 1.064 1.086 1.044
1.109
1.5ng/mL 0.685 0.701 0.659
0.717
0.75ng/mL 0.404 0.416 0.373
0.428
0ng/mL 0.051 0.043 0
0.035

Precision

We measured random samples of E1841Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.6 0.12 7.6
Intra-Assay 2 18 3.1 0.22 6.9
Intra-Assay 3 18 6.6 0.34 5.1

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1841Ra

    $320.00

  • 96T

    E1841Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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