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Home > Product > ELISA Kit > Rat Activity-regulated Cytoskeleton-associated Protein, ARC ELISA Kit

Rat Activity-regulated Cytoskeleton-associated Protein, ARC ELISA Kit

Rat Activity-regulated Cytoskeleton-associated Protein, ARC ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.48ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.48ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Activity-Regulated Cytoskeleton-Associated Protein

Code

E1842Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.48ng/L

Detection range

1-400ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system (PubMed: 29328916). ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation (PubMed: 29328916). ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons (PubMed: 29328916). Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory (PubMed: 10818134, PubMed: 17088211, PubMed: 17088212, PubMed: 17088213). Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis (PubMed: 17088211, PubMed: 17088212). Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses (PubMed: 23791196). Accumulates at weaker synapses, probably to prevent their undesired enhancement (PubMed: 22579289). This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material (PubMed: 29328916). Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory (PubMed: 10818134). Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1 (By similarity). In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation (By similarity).

UniProt accession

MASS(Da)

45,353

GeneID

54323

Synonyms

Activity-regulated cytoskeleton-associated protein;Activity-regulated gene 3.1 protein;ARC;ARC/ARG3.1;Arg3.1

Gene names

ARC

Research area

Neuroscience

Target protein

ARC

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1842Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/L 2.146 2.232 2.185
2.319
120ng/L 1.131 1.216 1.168
1.3
60ng/L 0.832 0.774 0.726
0.715
30ng/L 0.462 0.433 0.386
0.403
15ng/L 0.199 0.187 0.139
0.174
0ng/L 0.055 0.047 0
0.039

Precision

We measured random samples of E1842Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 84.8 4.2 5
Intra-Assay 2 18 176.4 5.66 3.2
Intra-Assay 3 18 52.5 3.37 6.4

Protocols

References

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  • Size

    Code

    Price

  • 48T

    E1842Ra

    $320.00

  • 96T

    E1842Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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