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Home > Product > ELISA Kit > Rat Contactin-1, CNTN1 ELISA Kit

Rat Contactin-1, CNTN1 ELISA Kit

Rat Contactin-1, CNTN1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.048ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.048ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Contactin-1

Code

E1927Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.048ng/mL

Detection range

0.1-40ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.

UniProt accession

MASS(Da)

113,495

GeneID

117258

Synonyms

CNTN 1;CNTN1;Contactin 1;Contactin-1;Neural cell surface protein F3

Gene names

CNTN1

Research area

Neuroscience

Target protein

CNTN1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1927Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/mL 2.268 2.269 2.236
2.27
12ng/mL 1.26 1.238 1.205
1.216
6ng/mL 0.67 0.702 0.669
0.733
3ng/mL 0.385 0.392 0.359
0.398
1.5ng/mL 0.165 0.145 0.112
0.125
0ng/mL 0.035 0.033 0
0.03

Precision

We measured random samples of E1927Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.5 0.35 4.7
Intra-Assay 2 18 15.8 0.48 3
Intra-Assay 3 18 4.3 0.27 6.1

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E1927Ra

    $320.00

  • 96T

    E1927Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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