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Home > Product > ELISA Kit > Rat Cubilin, CUBN ELISA Kit

Rat Cubilin, CUBN ELISA Kit

Rat Cubilin, CUBN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.53ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 7.53ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Cubilin

Code

E1933Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.53ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Endocytic receptor which plays a role in lipoprotein, vitamin and iron metabolism by facilitating their uptake. Acts together with LRP2 to mediate endocytosis of high-density lipoproteins, GC, hemoglobin, ALB, TF and SCGB1A1. Acts together with AMN to mediate endocytosis of the CBLIF-cobalamin complex. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the CBLIF-cobalamin complex. Ligand binding requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface.

UniProt accession

MASS(Da)

398,987

GeneID

80848

Synonyms

460 kDa receptor;Cubilin;CUBN;Glycoprotein 280;gp280;Intrinsic factor-cobalamin receptor;Intrinsic factor-vitamin B12 receptor

Gene names

CUBN

Research area

Others

Target protein

CUBN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1933Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.172 2.268 2.23
2.364
800ng/L 1.24 1.295 1.257
1.349
400ng/L 0.827 0.778 0.74
0.728
200ng/L 0.422 0.413 0.375
0.404
100ng/L 0.169 0.171 0.133
0.172
0ng/L 0.049 0.038 0
0.027

Precision

We measured random samples of E1933Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 638.8 27.78 4.3
Intra-Assay 2 18 883.5 27.51 3.1
Intra-Assay 3 18 252.1 13.04 5.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1933Ra

    $320.00

  • 96T

    E1933Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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