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Home > Product > ELISA Kit > Rat Cystathionine Β-synthase, CBS ELISA Kit

Rat Cystathionine Β-synthase, CBS ELISA Kit

Rat Cystathionine Β-synthase, CBS ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.13ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 9.13ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Cystathionine beta-Synthase

Code

E1937Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.13ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (By similarity). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (PubMed: 201$498.00|$360.0043, PubMed: 8558235).

UniProt accession

MASS(Da)

61,455

GeneID

24250

Synonyms

Beta-thionase;CBS;Cystathionine beta-synthase;Hemoprotein H-450;Serine sulfhydrase

Gene names

CBS

Research area

Others

Target protein

CBS

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1937Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.03 2.09 2.061
2.15
1200ng/L 1.734 1.633 1.604
1.532
600ng/L 1.113 1.151 1.122
1.189
300ng/L 0.72 0.679 0.65
0.637
150ng/L 0.431 0.463 0.435
0.496
0ng/L 0.031 0.029 0
0.027

Precision

We measured random samples of E1937Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 635.9 45.46 7.1
Intra-Assay 2 18 1457.7 111.24 7.6
Intra-Assay 3 18 132.5 9.15 6.9

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1937Ra

    $320.00

  • 96T

    E1937Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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