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Home > Product > ELISA Kit > Rat Fractalkine, FK ELISA Kit

Rat Fractalkine, FK ELISA Kit

Rat Fractalkine, FK ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.053ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.053ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Fractalkine

Code

E1968Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.053ng/mL

Detection range

0.1-40ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 and to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium.

UniProt accession

MASS(Da)

41,977

GeneID

89808

Synonyms

CX3C membrane-anchored chemokine;C-X3-C motif chemokine 1;CX3CL1;Fractalkine;Neurotactin;Processed fractalkine;Small-inducible cytokine D1

Gene names

CX3CL1

Research area

Cardiovascular

Target protein

CX3CL1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1968Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/mL 2.072 2.13 2.078
2.187
12ng/mL 1.528 1.471 1.419
1.415
6ng/mL 0.886 0.808 0.756
0.73
3ng/mL 0.488 0.469 0.417
0.45
1.5ng/mL 0.276 0.263 0.211
0.25
0ng/mL 0.07 0.052 0
0.034

Precision

We measured random samples of E1968Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 16.2 0.67 4.1
Intra-Assay 2 18 8.5 0.49 5.8
Intra-Assay 3 18 2.7 0.13 4.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1968Ra

    $320.00

  • 96T

    E1968Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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