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Home > Product > ELISA Kit > Rat Glypican 1, GPC1 ELISA Kit

Rat Glypican 1, GPC1 ELISA Kit

Rat Glypican 1, GPC1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 34.14ng/L

Detection range: 70-15000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 34.14ng/L

Detection range: 70-15000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Glypican 1

Code

E1993Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

34.14ng/L

Detection range

70-15000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Cell surface proteoglycan that bears heparan sulfate. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. Binds Cu(2+) or Zn(2+) ions (By similarity). Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination.

UniProt accession

MASS(Da)

61,734

GeneID

58920

Synonyms

Glypican-1;GPC 1;GPC1;HSPG M12;Secreted glypican-1

Gene names

GPC1

Research area

Others

Target protein

GPC1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1993Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8000ng/L 2.248 2.315 2.273
2.381
4000ng/L 1.362 1.265 1.223
1.168
2000ng/L 0.648 0.661 0.619
0.673
1000ng/L 0.457 0.42 0.378
0.383
500ng/L 0.176 0.188 0.146
0.201
0ng/L 0.036 0.042 0
0.049

Precision

We measured random samples of E1993Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1674.4 112.43 6.7
Intra-Assay 2 18 5242.7 175.07 3.3
Intra-Assay 3 18 2353 107.45 4.6

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E1993Ra

    $320.00

  • 96T

    E1993Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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