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Home > Product > ELISA Kit > Human Toll-like Receptor 3, TLR3 ELISA Kit

Human Toll-like Receptor 3, TLR3 ELISA Kit

Human Toll-like Receptor 3, TLR3 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 24.94ng/L

Detection range: 50-10000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 24.94ng/L

Detection range: 50-10000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Toll-Like Receptor 3

Code

E0365Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

24.94ng/L

Detection range

50-10000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response.

UniProt accession

MASS(Da)

103,829

GeneID

7098

Synonyms

CD antigen CD283;CD283;TLR 3;TLR3;Toll-like receptor 3

Gene names

TLR3

Research area

Immunology; Microbiology; Signal Transduction

Target protein

TLR3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0365Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6400ng/L 2.29 2.227 2.182
2.163
3200ng/L 1.294 1.262 1.218
1.23
1600ng/L 0.807 0.798 0.754
0.789
800ng/L 0.405 0.447 0.402
0.488
400ng/L 0.278 0.297 0.253
0.316
0ng/L 0.043 0.044 0
0.046

Precision

We measured random samples of E0365Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4903.2 177.35 3.6
Intra-Assay 2 18 1529.3 98.38 6.4
Intra-Assay 3 18 1537.1 107.77 7

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 96T

    E0365Hu

    $458.00

  • 48T

    E0365Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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