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Home > Product > ELISA Kit > Human Complement 3, C3 ELISA Kit

Human Complement 3, C3 ELISA Kit

Human Complement 3, C3 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.025mg/ml

Detection range: 0.05-30mg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.025mg/ml

Detection range: 0.05-30mg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Complement 3

Code

E0373Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.025mg/ml

Detection range

0.05-30mg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. [C3-beta-c]: Acts as a chemoattractant for neutrophils in chronic inflammation. [Acylation stimulating protein]: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed: 8376604, PubMed: 2909530, PubMed: 9059512, PubMed: 10432298, PubMed: 15833747, PubMed: 16333141, PubMed: 19615750).

UniProt accession

MASS(Da)

187,148

GeneID

718

Synonyms

Acylation stimulating protein;ASP;C3;C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1;C3a anaphylatoxin;C3adesArg;C3bc;C3-beta-c;Complement C3;Complement C3 alpha chain;Complement C3 beta chain;Complement C3b alpha' chain;Complement C3c alpha' chain fragment 1;Complement C3c alpha' chain fragment 2;Complement C3d fragment;Complement C3dg fragment;Complement C3f fragment;Complement C3g fragment

Gene names

C3

Research area

Cancer

Target protein

C3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0373Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16mg/ml 2.418 2.332 2.298
2.247
8mg/ml 1.369 1.295 1.26
1.221
4mg/ml 0.691 0.704 0.669
0.718
2mg/ml 0.44 0.442 0.407
0.444
1mg/ml 0.121 0.155 0.12
0.188
0mg/ml 0.017 0.035 0
0.052

Precision

We measured random samples of E0373Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.9 0.23 3.9
Intra-Assay 2 18 8.9 0.28 3.2
Intra-Assay 3 18 4 0.21 5.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 96T

    E0373Hu

    $458.00

  • 48T

    E0373Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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