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Home > Product > ELISA Kit > Human Aryl Hydrocarbon Receptor, AHR ELISA Kit

Human Aryl Hydrocarbon Receptor, AHR ELISA Kit

Human Aryl Hydrocarbon Receptor, AHR ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 5.25ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 5.25ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Aryl Hydrocarbon Receptor

Code

E0664Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

5.25ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Ligand-activated transcription factor that enables cells to adapt to changing conditions by sensing compounds from the environment, diet, microbiome and cellular metabolism, and which plays important roles in development, immunity and cancer (PubMed: 30373764, PubMed: 23275542, PubMed: 7961644, PubMed: 32818467). Upon ligand binding, translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE) (PubMed: 30373764, PubMed: 23275542, PubMed: 7961644). Regulates a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (PubMed: 12213388). Xenobiotics can act as ligands: upon xenobiotic-binding, activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene) (PubMed: 7961644). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons (PubMed: 7961644). Next to xenobiotics, natural ligands derived from plants, microbiota, and endogenous metabolism are potent AHR agonists (PubMed: 18076143). Tryptophan (Trp) derivatives constitute an important class of endogenous AHR ligands (PubMed: 32866000, PubMed: 32818467). Acts as a negative regulator of anti-tumor immunity: indoles and kynurenic acid generated by Trp catabolism act as ligand and activate AHR, thereby promoting AHR-driven cancer cell motility and suppressing adaptive immunity (PubMed: 32818467). Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1 (PubMed: 28602820). Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1 (PubMed: 28602820). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed: 28602820).

UniProt accession

MASS(Da)

96,147

GeneID

196

Synonyms

Ah receptor;AHR;Aryl hydrocarbon receptor;bHLHe76;Class E basic helix-loop-helix protein 76

Gene names

AHR

Research area

Epigenetics and Nuclear Signaling

Target protein

AHR

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0664Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.389 2.354 2.283
2.318
600ng/L 1.202 1.319 1.248
1.435
300ng/L 0.82 0.817 0.746
0.814
150ng/L 0.447 0.431 0.361
0.415
75ng/L 0.269 0.276 0.205
0.283
0ng/L 0.067 0.071 0
0.074

Precision

We measured random samples of E0664Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 209.4 10.43 5
Intra-Assay 2 18 404.8 20.52 5.1
Intra-Assay 3 18 638.3 20.98 3.3

Protocols

References

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  • Size

    Code

    Price

  • 96T

    E0664Hu

    $458.00

  • 48T

    E0664Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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