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Home > Product > ELISA Kit > Human Cystathionine-gamma-lyase, CSE ELISA Kit

Human Cystathionine-gamma-lyase, CSE ELISA Kit

Human Cystathionine-gamma-lyase, CSE ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.12ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.12ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cystathionine-Gamma-Lyase

Code

E0724Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.12ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.

UniProt accession

MASS(Da)

44,508

GeneID

1491

Synonyms

CTH;Cystathionine gamma-lyase;Cysteine-protein sulfhydrase;Gamma-cystathionase

Gene names

CTH

Research area

Others

Target protein

CTH

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Cystathionine-gamma-lyase, CSE ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.104 2.113 2.055
2.123
20ng/ml 1.226 1.283 1.226
1.341
10ng/ml 0.826 0.817 0.759
0.807
5ng/ml 0.479 0.451 0.393
0.422
2.5ng/ml 0.309 0.274 0.217
0.24
0ng/ml 0.04 0.058 0
0.076

Precision

We measured random samples of E0724Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 8.3 0.57 6.8
Intra-Assay 2 18 21.7 0.67 3.1
Intra-Assay 3 18 10.8 0.63 5.8

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 96T

    $458.00

  • 48T

    $320.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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