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Home > Product > ELISA Kit > Human Adenosine Deaminase, ADA ELISA Kit

Human Adenosine Deaminase, ADA ELISA Kit

Human Adenosine Deaminase, ADA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.05ng/ml

Detection range: 0.1-40.ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.05ng/ml

Detection range: 0.1-40.ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Adenosine Deaminase

Code

E0777Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.05ng/ml

Detection range

0.1-40.ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine (PubMed: 8452534, PubMed: 16670267). Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4 (PubMed: 20959412). Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (PubMed: 11772392). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation (PubMed: 20959412). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (PubMed: 23193172). Stimulates plasminogen activation (PubMed: 15016824). Plays a role in male fertility (PubMed: 21919946, PubMed: 26166670). Plays a protective role in early postimplantation embryonic development (By similarity).

UniProt accession

MASS(Da)

40,764

GeneID

100

Synonyms

ADA;Adenosine aminohydrolase;Adenosine deaminase

Gene names

ADA

Research area

Epigenetics and Nuclear Signaling

Target protein

ADA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0777Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.616 2.581 2.524
2.545
12ng/ml 1.455 1.406 1.349
1.357
6ng/ml 0.878 0.856 0.799
0.835
3ng/ml 0.434 0.46 0.403
0.487
1.5ng/ml 0.246 0.235 0.178
0.224
0ng/ml 0.063 0.057 0
0.051

Precision

We measured random samples of E0777Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 14.5 0.48 3.3
Intra-Assay 2 18 9.3 0.48 5.1
Intra-Assay 3 18 5.4 0.33 6.1

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0777Hu

    $320.00

  • 96T

    E0777Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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