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Home > Product > ELISA Kit > Human 5-Nucleotidase, 5-NT ELISA Kit

Human 5-Nucleotidase, 5-NT ELISA Kit

Human 5-Nucleotidase, 5-NT ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.34ng/mL

Detection range: 0.5-150ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.34ng/mL

Detection range: 0.5-150ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

5-Nucleotidase

Code

E0831Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.34ng/mL

Detection range

0.5-150ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

UniProt accession

MASS(Da)

63,368

GeneID

4907

Synonyms

5'-NT;5'-nucleotidase;CD antigen CD73;Ecto-5'-nucleotidase;NT5E

Gene names

NT5E

Research area

Immunology

Target protein

NT5E

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0831Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
80ng/mL 2.055 2.091 2.066
2.127
40ng/mL 1.513 1.407 1.382
1.3
20ng/mL 0.758 0.688 0.663
0.618
10ng/mL 0.4 0.401 0.376
0.401
5ng/mL 0.212 0.209 0.184
0.206
0ng/mL 0.024 0.025 0
0.026

Precision

We measured random samples of E0831Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 23.5 1 4.2
Intra-Assay 2 18 41.2 1.41 3.4
Intra-Assay 3 18 12.2 0.8 6.5

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0831Hu

    $320.00

  • 96T

    E0831Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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