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Home > Product > ELISA Kit > Human Glucose-6-phosphate Isomerase, GPI ELISA Kit

Human Glucose-6-phosphate Isomerase, GPI ELISA Kit

Human Glucose-6-phosphate Isomerase, GPI ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.51ng/ml

Detection range: 7-1500ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 3.51ng/ml

Detection range: 7-1500ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Glucose-6-Phosphate Isomerase

Code

E0887Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.51ng/ml

Detection range

7-1500ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed: 28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed: 11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed: 3352745, PubMed: 11004567). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed: 3352745, PubMed: 11004567).

UniProt accession

MASS(Da)

63,147

GeneID

2821

Synonyms

AMF;Autocrine motility factor;Glucose-6-phosphate isomerase;GPI;Neuroleukin;NLK;PGI;PHI;Phosphoglucose isomerase;Phosphohexose isomerase;SA-36;Sperm antigen 36

Gene names

GPI

Research area

Metabolism

Target protein

GPI

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0887Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/ml 2.269 2.322 2.27
2.376
400ng/ml 1.789 1.749 1.696
1.708
200ng/ml 1.163 1.23 1.178
1.297
100ng/ml 0.604 0.666 0.613
0.727
50ng/ml 0.394 0.397 0.345
0.401
0ng/ml 0.056 0.052 0
0.048

Precision

We measured random samples of E0887Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 185.3 12.8 6.9
Intra-Assay 2 18 321.9 11.83 3.7
Intra-Assay 3 18 45.9 2.79 6.1

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0887Hu

    $320.00

  • 96T

    E0887Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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