Human Matrix Metalloproteinase 13, MMP13 ELISA Kit

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Home > Product > ELISA Kit > Human Matrix Metalloproteinase 13, MMP13 ELISA Kit

Human Matrix Metalloproteinase 13, MMP13 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.04ng/ml

Detection range: 0.1-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.04ng/ml

Detection range: 0.1-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Matrix Metalloproteinase 13

Code

E0911Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.04ng/ml

Detection range

0.1-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.

UniProt accession

P45452

MASS（Da）

53,820

GeneID

4322

Synonyms

Collagenase 3;Matrix Metalloproteinase-13;MMP 13;MMP13;MMP-13

Gene names

MMP13

Research area

Cardiovascular; Signal Transduction; Cancer

Target protein

MMP13

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E0911Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.21	2.163	2.117
	2.115
8ng/ml	1.322	1.367	1.321
	1.412
4ng/ml	0.832	0.868	0.822
	0.904
2ng/ml	0.519	0.478	0.432
	0.436
1ng/ml	0.243	0.263	0.217
	0.284
0ng/ml	0.036	0.046	0
	0.056

Precision

We measured random samples of E0911Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.7	0.22	6
Intra-Assay	2	18	11.1	0.29	2.6
Intra-Assay	3	18	2.1	0.13	6.5

Protocols

> Protocol Booklet

References (1)

Effect of MMP-13 Levels? on Disease Modifying Antirheumaticdrugs (DMARDS) and Corticosteroids on Rheumatoid Arthritis Patients With Chronic Periodontitis-A Biochemical Analysis

Journal: Biosciences Biotechnology Research Asia; Bhopal Vol. 14, Iss. 3, (Sep 2017): 1017-1024.

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Associated products

Datasheet

Size

Code

Price

48T

E0911Hu

$320.00
96T

E0911Hu

$458.00

Add to Cart

Lead time: Within two weeks

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