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Home > Product > ELISA Kit > Human Angiotensin Converting Enzyme, ACE ELISA Kit

Human Angiotensin Converting Enzyme, ACE ELISA Kit

Human Angiotensin Converting Enzyme, ACE ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.56U/L

Detection range: 1-400U/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.56U/L

Detection range: 1-400U/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Angiotensin Converting Enzyme

Code

E0927Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.56U/L

Detection range

1-400U/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.

UniProt accession

MASS(Da)

149,715

GeneID

1636

Synonyms

ACE;Angiotensin-converting enzyme;Angiotensin-converting enzyme, soluble form;CD antigen CD143;Dipeptidyl carboxypeptidase I;Kininase II

Gene names

ACE

Research area

Cardiovascular; Signal Transduction; Cell Biology; Cardiovascular; Cancer; Metabolism

Target protein

ACE

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0927Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240U/L 1.906 1.887 1.843
1.868
120U/L 0.939 1.007 0.963
1.075
60U/L 0.552 0.533 0.488
0.513
30U/L 0.289 0.226 0.182
0.163
15U/L 0.081 0.084 0.039
0.086
0U/L 0.028 0.044 0
0.061

Precision

We measured random samples of E0927Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 86 4.79 5.6
Intra-Assay 2 18 218.3 7.53 3.4
Intra-Assay 3 18 113 4.22 3.7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0927Hu

    $320.00

  • 96T

    E0927Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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