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Home > Product > ELISA Kit > Human Pulmonary Surfatcant-associated Protein B, SP-B ELISA Kit

Human Pulmonary Surfatcant-associated Protein B, SP-B ELISA Kit

Human Pulmonary Surfatcant-associated Protein B, SP-B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.12ng/ml

Detection range: 2-600ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 1.12ng/ml

Detection range: 2-600ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Pulmonary Surfatcant-Associated Protein B

Code

E1069Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.12ng/ml

Detection range

2-600ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

UniProt accession

MASS(Da)

42,117

GeneID

6439

Synonyms

18 kDa pulmonary-surfactant protein;6 kDa protein;Pulmonary surfactant-associated protein B;Pulmonary surfactant-associated proteolipid SPL(Phe;SFTPB;SP-B

Gene names

SFTPB

Research area

Signal Transduction

Target protein

SFTPB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1069Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
320ng/ml 2.281 2.34 2.27
2.4
160ng/ml 1.216 1.326 1.256
1.436
80ng/ml 0.761 0.828 0.758
0.896
40ng/ml 0.549 0.532 0.461
0.514
20ng/ml 0.319 0.279 0.208
0.239
0ng/ml 0.062 0.07 0
0.078

Precision

We measured random samples of E1069Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 131.4 7.98 6.1
Intra-Assay 2 18 181.9 5.69 3.1
Intra-Assay 3 18 68.6 5.18 7.5

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1069Hu

    $320.00

  • 96T

    E1069Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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