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Home > Product > ELISA Kit > Human Angiopoietin 2, ANG-2 ELISA Kit

Human Angiopoietin 2, ANG-2 ELISA Kit

Human Angiopoietin 2, ANG-2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.49ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.49ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Angiopoietin 2

Code

E1221Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.49ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

UniProt accession

MASS(Da)

56,919

GeneID

285

Synonyms

ANG 2;ANG-2;Angiopoietin-2

Gene names

ANG-2

Research area

Cardiovascular

Target protein

ANG-2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1221Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 1.945 1.825 1.77
1.706
320ng/L 1.061 1.041 0.986
1.021
160ng/L 0.535 0.567 0.512
0.599
80ng/L 0.289 0.237 0.182
0.185
40ng/L 0.135 0.12 0.065
0.106
0ng/L 0.059 0.056 0
0.052

Precision

We measured random samples of E1221Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 528.7 16.52 3.1
Intra-Assay 2 18 201.8 9.55 4.7
Intra-Assay 3 18 354.8 18.19 5.1

Protocols

References (2)

Effect of Vitamin E on Serum Levels of Vascular Endothelial Growth Factor and Angiopoietin-1 in Women with Polycystic Ovary Syndrome: A Pilot Randomized, Placebo-Controlled Trial.
Angiopoietin-2 as a predictor of fibrosis regression in chronic hepatitis C virus patients after direct-acting antiviral drugs

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  • Size

    Code

    Price

  • 48T

    E1221Hu

    $320.00

  • 96T

    E1221Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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