Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action. [provided by RefSeq, Jul 2008]
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1269Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 32ng/ml | 1.72 | 1.768 | 1.728 |
| 1.816 | |||
| 16ng/ml | 1.006 | 1.001 | 0.961 |
| 0.996 | |||
| 8ng/ml | 0.539 | 0.589 | 0.549 |
| 0.638 | |||
| 4ng/ml | 0.201 | 0.176 | 0.136 |
| 0.151 | |||
| 2ng/ml | 0.126 | 0.117 | 0.078 |
| 0.109 | |||
| 0ng/ml | 0.029 | 0.04 | 0 |
| 0.051 |
Precision
We measured random samples of E1269Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 9.3 | 0.43 | 4.6 |
| Intra-Assay | 2 | 18 | 15.1 | 0.72 | 4.7 |
| Intra-Assay | 3 | 18 | 32.1 | 1.65 | 5.1 |
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