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Home > Product > ELISA Kit > Bovine T-cell Surface Glycoprotein Cd8 Alpha Chain, CD8A ELISA Kit

Bovine T-cell Surface Glycoprotein Cd8 Alpha Chain, CD8A ELISA Kit

Bovine T-cell Surface Glycoprotein Cd8 Alpha Chain, CD8A ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.91ng/ml

Detection range: 2-600ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 0.91ng/ml

Detection range: 2-600ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

T-Cell Surface Glycoprotein Cd8 Alpha Chain

Code

E0326Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.91ng/ml

Detection range

2-600ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I Peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

UniProt accession

MASS(Da)

26,417

GeneID

281060

Synonyms

CD 8A;CD antigen CD8a;CD8A;T-cell surface glycoprotein CD8 alpha chain

Gene names

CD8A

Research area

Immunology; Cancer

Target protein

CD8A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Bovine T-cell Surface Glycoprotein Cd8 Alpha Chain, CD8A ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400ng/ml 2.311 2.287 2.223
2.263
200ng/ml 1.863 1.835 1.771
1.807
100ng/ml 1.186 1.213 1.148
1.24
50ng/ml 0.68 0.699 0.635
0.718
25ng/ml 0.386 0.4 0.336
0.415
0ng/ml 0.066 0.064 0
0.062

Precision

We measured random samples of E0326Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 81.6 4.47 5.5
Intra-Assay 2 18 162.2 7.97 4.9
Intra-Assay 3 18 40 2.43 6.1

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 96T

    $458.00

  • 48T

    $320.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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