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Home > Product > ELISA Kit > Human Adiponectin, ADP ELISA Kit

Human Adiponectin, ADP ELISA Kit

Human Adiponectin, ADP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.11mg/L

Detection range: 0.2-60mg/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.11mg/L

Detection range: 0.2-60mg/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Adiponectin

Code

E1550Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.11mg/L

Detection range

0.2-60mg/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

UniProt accession

MASS(Da)

26,414

GeneID

9370

Synonyms

30 kDa adipocyte complement-related protein;ACRP30;Adipocyte complement-related 30 kDa protein;Adipocyte, C1q and collagen domain-containing protein;Adiponectin;Adipose most abundant gene transcript 1 protein;APM 1;APM1;apM-1;Gelatin-binding protein

Gene names

APM1

Research area

Others

Target protein

APM1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1550Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32mg/L 1.768 1.87 1.837
1.973
16mg/L 1.092 1.037 1.004
0.983
8mg/L 0.557 0.553 0.519
0.548
4mg/L 0.175 0.206 0.173
0.237
2mg/L 0.115 0.115 0.082
0.115
0mg/L 0.027 0.033 0
0.039

Precision

We measured random samples of E1550Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 14.6 0.74 5.1
Intra-Assay 2 18 30.6 1.31 4.3
Intra-Assay 3 18 5.7 0.27 4.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E1550Hu

    $320.00

  • 96T

    E1550Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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