Tel:86 021 3100 7137

Share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human G1/S-specific Cyclin-D1, CYCLIN-D1 ELISA Kit

Human G1/S-specific Cyclin-D1, CYCLIN-D1 ELISA Kit

Human G1/S-specific Cyclin-D1, CYCLIN-D1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.052ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.052ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

G1/S-Specific Cyclin-D1

Code

E1636Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.052ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.

UniProt accession

MASS(Da)

33,729

GeneID

595

Synonyms

B-cell lymphoma 1 protein;BCL-1;BCL-1 oncogene;CCND 1;CCND1;G1/S-specific cyclin-D1;PRAD1 oncogene

Gene names

CCND1

Research area

Others

Target protein

CCND1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1636Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.384 2.29 2.245
2.196
12ng/ml 1.128 1.143 1.098
1.159
6ng/ml 0.826 0.806 0.76
0.786
3ng/ml 0.469 0.49 0.445
0.511
1.5ng/ml 0.245 0.245 0.199
0.244
0ng/ml 0.05 0.045 0
0.041

Precision

We measured random samples of E1636Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 16 0.54 3.4
Intra-Assay 2 18 11 0.62 5.7
Intra-Assay 3 18 4.7 0.3 6.5

Protocols

References

Customer reviews and Q&As

No reviews
No reviews

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up