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Home > Product > ELISA Kit > Human Atp-sensitive Inward Rectifier Potassium Channel 10, KCNJ10 ELISA Kit

Human Atp-sensitive Inward Rectifier Potassium Channel 10, KCNJ10 ELISA Kit

Human Atp-sensitive Inward Rectifier Potassium Channel 10, KCNJ10 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.32ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.32ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Atp-Sensitive Inward Rectifier Potassium Channel 10

Code

E1794Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.32ng/L

Detection range

20-3800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium Channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the Channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity). In the kidney, together with KCNJ16, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules.

UniProt accession

MASS(Da)

42,508

GeneID

3766

Synonyms

ATP-dependent inwardly rectifying potassium Channel Kir4.1;ATP-sensitive inward rectifier potassium Channel 10;Inward rectifier K(+ Channel Kir1.2;KCNJ 10;KCNJ10;Potassium Channel, inwardly rectifying subfamily J member 10

Gene names

KCNJ10

Research area

Neuroscience

Target protein

KCNJ10

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1794Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.264 2.274 2.22
2.284
1000ng/L 1.331 1.343 1.289
1.355
500ng/L 0.819 0.782 0.728
0.746
250ng/L 0.418 0.437 0.383
0.456
125ng/L 0.328 0.288 0.234
0.248
0ng/L 0.054 0.054 0
0.054

Precision

We measured random samples of E1794Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1244.2 44.82 3.6
Intra-Assay 2 18 795.4 45.24 5.7
Intra-Assay 3 18 481.1 28.88 6

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E1794Hu

    $320.00

  • 96T

    E1794Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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