1,25-Hydroxyvitamin D

E1919Hu

Sandwich

48T,96T

3.14pmol/L

7-1500pmol/L

Serum, plasma, cell culture supernates

Human

2-8ºC

1h 30m

A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)) (PubMed: 24893882, PubMed: 15574355, PubMed: 8679605, PubMed: 11012668, PubMed: 16617161, PubMed: 29461981). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid (PubMed: 24893882, PubMed: 15574355). With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product (PubMed: 11012668, PubMed: 8679605). Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol (PubMed: 25727742). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed: 8679605).

58,875

1591

1,25-dihydroxyvitamin D,3 24-hydroxylase, mitochondrial;24-OHase;CYP 24A1;CYP24A1;Cytochrome P450 24A1;Cytochrome P450-CC24;Vitamin D, 3 24-hydroxylase

CYP24A1

Cancer

CYP24A1