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Home > Product > ELISA Kit > Human Beta-defensin 1, DEFB1 ELISA Kit

Human Beta-defensin 1, DEFB1 ELISA Kit

Human Beta-defensin 1, DEFB1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.31pg/ml

Detection range: 5-2000pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.31pg/ml

Detection range: 5-2000pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Beta-Defensin 1

Code

E1935Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.31pg/ml

Detection range

5-2000pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed: 25122636).

UniProt accession

MASS(Da)

7,420

GeneID

1672

Synonyms

BD-1;Beta-defensin 1;DEFB 1;DEFB1;Defensin, beta 1;hBD-1

Gene names

DEFB1

Research area

Microbiology

Target protein

DEFB1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1935Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200pg/ml 2.312 2.336 2.3
2.36
600pg/ml 1.732 1.771 1.735
1.811
300pg/ml 1.202 1.157 1.121
1.111
150pg/ml 0.618 0.675 0.639
0.733
75pg/ml 0.42 0.391 0.354
0.361
0pg/ml 0.03 0.036 0
0.043

Precision

We measured random samples of E1935Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 153.3 9.66 6.3
Intra-Assay 2 18 236.5 14.17 6
Intra-Assay 3 18 362.7 16.79 4.6

Protocols

References (1)

Serum human β-defensin-1 (hBD-1) and -20G/A DEFB1 gene polymorphism in Behçet’s disease patients: Relation to clinical characteristics

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  • Size

    Code

    Price

  • 48T

    E1935Hu

    $320.00

  • 96T

    E1935Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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