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Home > Product > ELISA Kit > Human Bone Morphogenetic Protein 2, BMP-2 ELISA Kit

Human Bone Morphogenetic Protein 2, BMP-2 ELISA Kit

Human Bone Morphogenetic Protein 2, BMP-2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.01ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.01ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Bone Morphogenetic Protein 2

Code

E1989Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.01ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Induces cartilage and bone formation (PubMed: 3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed: 24362451).

UniProt accession

MASS(Da)

44,702

GeneID

650

Synonyms

BMP 2;BMP2;BMP-2;BMP-2A;Bone morphogenetic protein 2;Bone morphogenetic protein 2A

Gene names

BMP2

Research area

Signal Transduction

Target protein

BMP2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1989Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.113 2.035 1.996
1.957
4ng/ml 1.146 1.1 1.061
1.055
2ng/ml 0.607 0.609 0.57
0.611
1ng/ml 0.192 0.231 0.192
0.27
0.5ng/ml 0.127 0.127 0.088
0.126
0ng/ml 0.029 0.039 0
0.049

Precision

We measured random samples of E1989Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.4 0.05 3.6
Intra-Assay 2 18 5.9 0.15 2.6
Intra-Assay 3 18 3.7 0.17 4.6

Protocols

References (1)

Investigating biological impact of HAp from goat femur reinforced with Zr–Ag for bone tissue engineering application

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  • Size

    Code

    Price

  • 48T

    E1989Hu

    $320.00

  • 96T

    E1989Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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