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Home > Product > ELISA Kit > Human Ryanodine Receptor 1, RYR1 ELISA Kit

Human Ryanodine Receptor 1, RYR1 ELISA Kit

Human Ryanodine Receptor 1, RYR1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.05ng/ml

Detection range: 0.1-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.05ng/ml

Detection range: 0.1-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ryanodine Receptor 1

Code

E1992Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.05ng/ml

Detection range

0.1-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Calcium Channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules (PubMed: 11741831, PubMed: 16163667). Repeated very high-level exercise increases the open probability of the Channel and leads to Ca(2+) leaking into the cytoplasm (PubMed: 18268335). Can also mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis (By similarity).

UniProt accession

MASS(Da)

565,176

GeneID

6261

Synonyms

Ryanodine receptor 1;RYR 1;RYR1;RYR-1;Skeletal muscle calcium release Channel;Skeletal muscle ryanodine receptor;Skeletal muscle-type ryanodine receptor;Type 1 ryanodine receptor

Gene names

RYR1

Research area

Others

Target protein

RYR1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1992Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.206 2.16 2.101
2.113
8ng/ml 1.444 1.372 1.314
1.3
4ng/ml 0.938 0.937 0.878
0.935
2ng/ml 0.485 0.519 0.461
0.553
1ng/ml 0.302 0.295 0.237
0.289
0ng/ml 0.069 0.058 0
0.047

Precision

We measured random samples of E1992Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.5 0.29 6.5
Intra-Assay 2 18 7.5 0.29 3.9
Intra-Assay 3 18 1.3 0.09 7

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E1992Hu

    $320.00

  • 96T

    E1992Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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