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Home > Product > ELISA Kit > Human Growth Differentiation Factor 5, GDF5 ELISA Kit

Human Growth Differentiation Factor 5, GDF5 ELISA Kit

Human Growth Differentiation Factor 5, GDF5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Growth Differentiation Factor 5

Code

E2268Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.026ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (PubMed: 24098149, PubMed: 21976273, PubMed: 15530414, PubMed: 25092592). Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (PubMed: 21976273). Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (By similarity). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed: 11276205).

UniProt accession

MASS(Da)

55,411

GeneID

8200

Synonyms

BMP-14;Bone morphogenetic protein 14;Cartilage-derived morphogenetic protein 1;CDMP-1;GDF 5;GDF5;GDF-5;Growth/differentiation factor 5;LAP-4;Lipopolysaccharide-associated protein 4;LPS-associated protein 4;Radotermin

Gene names

GDF5

Research area

Signal Transduction

Target protein

GDF5

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2268Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 1.665 1.679 1.634
1.693
6ng/ml 1.073 1.042 0.998
1.012
3ng/ml 0.419 0.436 0.392
0.454
1.5ng/ml 0.234 0.254 0.209
0.274
0.75ng/ml 0.107 0.084 0.04
0.062
0ng/ml 0.053 0.045 0
0.036

Precision

We measured random samples of E2268Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.7 0.35 5.2
Intra-Assay 2 18 8.9 0.39 4.3
Intra-Assay 3 18 2.5 0.11 4.5

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2268Hu

    $320.00

  • 96T

    E2268Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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