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Home > Product > ELISA Kit > Human Cyclin T1, CCNT1 ELISA Kit

Human Cyclin T1, CCNT1 ELISA Kit

Human Cyclin T1, CCNT1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.96ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.96ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cyclin T1

Code

E2283Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.96ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). (Microbial infection) In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.

UniProt accession

MASS(Da)

80,685

GeneID

904

Synonyms

CCNT 1;CCNT1;Cyclin-T;Cyclin-T1;CycT1

Gene names

CCNT1

Research area

Microbiology

Target protein

CCNT1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Cyclin T1, CCNT1 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 1.809 1.797 1.769
1.785
600ng/L 1.068 1.09 1.062
1.113
300ng/L 0.419 0.441 0.412
0.463
150ng/L 0.215 0.196 0.168
0.178
75ng/L 0.08 0.099 0.07
0.117
0ng/L 0.027 0.028 0
0.03

Precision

We measured random samples of E2283Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 618.2 28.9 4.7
Intra-Assay 2 18 1115.3 51.97 4.7
Intra-Assay 3 18 342.9 14.8 4.3

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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