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Home > Product > ELISA Kit > Human Adenomatosis Polyposis Coli Protein, APC ELISA Kit

Human Adenomatosis Polyposis Coli Protein, APC ELISA Kit

Human Adenomatosis Polyposis Coli Protein, APC ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.16ng/ml

Detection range: 2-700ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 1.16ng/ml

Detection range: 2-700ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Adenomatosis Polyposis Coli Protein

Code

E2284Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.16ng/ml

Detection range

2-700ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

UniProt accession

MASS(Da)

311,646

GeneID

324

Synonyms

Adenomatous polyposis coli protein;APC;Deleted in polyposis 2.5;Protein APC

Gene names

APC

Research area

Others

Target protein

APC

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2284Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400ng/ml 2.32 2.316 2.261
2.313
200ng/ml 1.434 1.397 1.341
1.36
100ng/ml 0.844 0.816 0.76
0.787
50ng/ml 0.453 0.444 0.388
0.434
25ng/ml 0.296 0.298 0.243
0.3
0ng/ml 0.044 0.055 0
0.067

Precision

We measured random samples of E2284Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 96.8 4.9 5.1
Intra-Assay 2 18 181 7.26 4
Intra-Assay 3 18 48.2 2.68 5.6

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2284Hu

    $320.00

  • 96T

    E2284Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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