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Home > Product > ELISA Kit > Human Claudin 1, CLDN1 ELISA Kit

Human Claudin 1, CLDN1 ELISA Kit

Human Claudin 1, CLDN1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.22ng/ml

Detection range: 0.5-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.22ng/ml

Detection range: 0.5-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Claudin 1

Code

E2300Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.22ng/ml

Detection range

0.5-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed: 23407391). (Microbial infection) Acts as a co-receptor for hepatitis C virus (HCV) in hepatocytes (PubMed: 17325668, PubMed: 20375010, PubMed: 24038151). Associates with CD81 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell (PubMed: 20375010). Acts as a receptor for dengue virus (PubMed: 24074594).

UniProt accession

MASS(Da)

22,744

GeneID

9076

Synonyms

Claudin-1;CLDN 1;CLDN1;Senescence-associated epithelial membrane protein

Gene names

CLDN1

Research area

Immunology

Target protein

CLDN1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2300Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.215 2.19 2.135
2.164
12ng/ml 1.498 1.416 1.361
1.333
6ng/ml 0.733 0.774 0.718
0.814
3ng/ml 0.501 0.471 0.416
0.442
1.5ng/ml 0.27 0.252 0.197
0.233
0ng/ml 0.061 0.055 0
0.049

Precision

We measured random samples of E2300Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 13 0.44 3.4
Intra-Assay 2 18 6.8 0.36 5.3
Intra-Assay 3 18 4.8 0.28 5.9

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2300Hu

    $320.00

  • 96T

    E2300Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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