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Home > Product > ELISA Kit > Human Catenin Beta 1, CTNNB1 ELISA Kit

Human Catenin Beta 1, CTNNB1 ELISA Kit

Human Catenin Beta 1, CTNNB1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.08ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.08ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Catenin Beta 1

Code

E2396Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.08ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Key downstream component of the canonical Wnt signaling pathway (PubMed: 17524503, PubMed: 18077326, PubMed: 18086858, PubMed: 18957423, PubMed: 21262353, PubMed: 22155184, PubMed: 22647378, PubMed: 22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed: 17524503, PubMed: 18077326, PubMed: 18086858, PubMed: 18957423, PubMed: 21262353, PubMed: 22155184, PubMed: 22647378, PubMed: 22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed: 17524503, PubMed: 18077326, PubMed: 18086858, PubMed: 18957423, PubMed: 21262353, PubMed: 22155184, PubMed: 22647378, PubMed: 22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed: 18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed: 21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed: 18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed: 22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity).

UniProt accession

MASS(Da)

85,497

GeneID

1499

Synonyms

Beta-catenin;Catenin beta-1;CTNNB 1;CTNNB1

Gene names

CTNNB1

Research area

Cardiovascular

Target protein

CTNNB1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2396Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 1.969 1.898 1.854
1.827
600ng/L 1.027 0.975 0.931
0.924
300ng/L 0.506 0.543 0.499
0.58
150ng/L 0.167 0.203 0.159
0.239
75ng/L 0.147 0.141 0.097
0.135
0ng/L 0.028 0.044 0
0.059

Precision

We measured random samples of E2396Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 524.9 25.95 4.9
Intra-Assay 2 18 933.6 21.99 2.4
Intra-Assay 3 18 412 19.76 4.8

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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