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Home > Product > ELISA Kit > Human Mitogen Activated Protein Kinase 1, MAP3K1 ELISA Kit

Human Mitogen Activated Protein Kinase 1, MAP3K1 ELISA Kit

Human Mitogen Activated Protein Kinase 1, MAP3K1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.55ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.55ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Mitogen Activated Protein kinase 1

Code

E2492Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.55ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Component of a protein kinase signal transduction cascade (PubMed: 9808624). Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4 (PubMed: 9808624). May phosphorylate the MAPK8/JNK1 kinase (PubMed: 17761173). Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway (PubMed: 9808624).

UniProt accession

MASS(Da)

164,470

GeneID

4214

Synonyms

MAP3K1;MAPK/ERK kinase 1;MEK kinase 1;MEKK 1;Mitogen-activated protein kinase kinase 1

Gene names

MAP3K1

Research area

Signal Transduction

Target protein

MAP3K1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2492Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.417 2.374 2.339
2.332
1200ng/L 1.396 1.305 1.269
1.213
600ng/L 0.728 0.727 0.691
0.725
300ng/L 0.316 0.336 0.3
0.356
150ng/L 0.165 0.188 0.153
0.211
0ng/L 0.049 0.036 0
0.022

Precision

We measured random samples of E2492Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 749.3 34.31 4.6
Intra-Assay 2 18 1713.5 46.18 2.7
Intra-Assay 3 18 351.2 15.54 4.4

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2492Hu

    $320.00

  • 96T

    E2492Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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